Part:BBa_K2010000:Experience

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Applications of BBa_K2010000

Here is TJUSLS_China(2021). We use PETase as our wild type as we want to improve the thermostability of PETase through mutations. We choose five methods to improve the thermostability of PETase, salt bridges, hydrogen bonds, hydrophilic interaction, prolines, and disulfide bonds. We tried to find out unstable regions in PETase by reading literature as well as doing structural analysis and visual screening via bioinformatics tools. Finally, we got 26 mutations which have 11-12 mutation sites each. And our best mutation-Super5 contains 11 mutation sites S214H-I168R-W159H-S188Q-R280A-A180I-G165A-Q119Y-L117F-T140D-S121E. Well, the enzyme activity and thermostability of Super5 have greatly improved 163 times，compared with PETase. If you want to know more detailed things about our project, please click this link to our parts page. Thanks a lot. https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2021&group=TJUSLS_China

User Reviews

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